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Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A
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Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A
Recombinant DNase I (RNase-free) is an endonuclease that catalyzes the random degradation of both single- and double-stranded DNA.
Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A
Item #: 84256292

Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A

Item #: 84256292

RWF 244079

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Recombinant DNase I (RNase-free) is an endonuclease that catalyzes the random degradation of both single- and double-stranded DNA.
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What Stands Out

High Purity
Recombinant DNase I is rigorously purified to ensure it is free from RNase contamination, making it ideal for sensitive applications in molecular biology, ensuring reliable results when handling RNA.
Broad Application
Effective in various applications, including nucleic acid purification, cell lysis, and DNA degradation, it enhances workflow efficiency in labs focused on genetics and microbiology.
Consistent Performance
Produced with strict quality controls, this enzyme offers consistent activity and reliability across experiments, ensuring reproducibility in results, which is crucial for scientific research and diagnostics.

Product Details

Shop Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A online at a best price in Rwanda. B07B438MWH
Item Weight0.5 lbs (230 grams)

Who Should Buy?

Suitable For
  • Molecular Biologists

    Ideal for researchers needing high-purity DNase for nucleic acid research and manipulation in experiments.

  • Cell Culture

    Beneficial for those working with sensitive cell lines to eliminate DNA contamination affecting growth.

  • Nucleic Acid Research

    Essential for projects involving the degradation of DNA for downstream applications in gene studies.

Not Suitable For
  • General Use

    Not suitable for general users without specific nucleic acid handling requirements or knowledge of enzyme usage.

  • Field Studies

    May not be appropriate for outdoor or non-controlled environments due to stability concerns and contamination risk.

  • Non-Research Applications

    Not recommend for applications outside of research, such as cooking or non-scientific use due to specific enzymatic properties.

Product Description

Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A

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Customer Questions & Answers

  • Question: What is Recombinant DNase I RNase-Free?

    Answer: Recombinant DNase I RNase-Free is an enzymatic product used for the degradation of DNA in various molecular biology applications. It is specifically designed to be free of RNase contamination, ensuring the integrity of RNA samples during experiments. It provides a potent means for removing unwanted DNA from RNA preparations. A common use case is in RNA purification protocols where the removal of genomic DNA is crucial for accurate gene expression analysis.
  • Question: How does Recombinant DNase I work?

    Answer: Recombinant DNase I functions by cleaving the phosphodiester bonds between nucleotides in DNA, resulting in short oligonucleotides. This action is essential in various applications, such as preparing samples for PCR or RT-PCR, where high DNA purity is required. The enzyme operates optimally at specific buffer conditions, making it vital to follow the recommended protocols for effective DNA degradation. For instance, during RNA work, using this enzyme helps ensure that downstream analyses are not affected by contaminating DNA.
  • Question: What are the applications of Recombinant DNase I RNase-Free?

    Answer: Recombinant DNase I RNase-Free is utilized in several key applications in molecular biology. It plays a crucial role in RNA purification, cell lysis, and removal of contaminating genomic DNA from RNA preparations. This enzyme is also used in cloning, sequencing, and hybridization studies. Additionally, researchers might use it in transfection protocols to enhance the quality of RNA samples. Each of these applications relies on the enzyme's ability to selectively degrade DNA, making it an indispensable tool in nucleic acid manipulation.
  • Question: Is Recombinant DNase I RNase-Free safe to use with RNA samples?

    Answer: Yes, Recombinant DNase I RNase-Free is specifically formulated to be free of RNase contamination, making it safe to use with RNA samples. The RNase-free formulation ensures that your RNA remains intact while efficiently degrading any DNA present. For example, researchers who are purifying RNA for qPCR or RT-PCR can utilize this enzyme without the risk of RNA degradation. Proper handling and adherence to usage guidelines will further ensure that your RNA samples provide accurate analytical results.
  • Question: How should Recombinant DNase I RNase-Free be stored?

    Answer: Recombinant DNase I RNase-Free should be stored at -20°C for long-term stability. When thawing, it is advisable to keep the enzyme on ice to maintain its activity. Frequent freeze-thaw cycles may reduce its effectiveness, so it is recommended to aliquot the enzyme into smaller volumes based on your experimental needs. This storage and handling approach helps ensure the enzyme remains potent for repeated use in various applications, from RNA purification to molecular cloning.
  • Question: Can Recombinant DNase I be used in all types of experiments?

    Answer: While Recombinant DNase I is versatile and widely applicable in molecular biology, it may not be suitable for all experiments. It is primarily used in situations where DNA elimination is necessary. For example, in RNA work, it is ideal for removing genomic DNA. However, if your experiment requires strict preservation of DNA, this enzyme would not be suitable. Always assess your specific research needs and select appropriate enzymes accordingly to achieve the desired outcomes of your experiments.
  • Question: What are the key features of Takara Bio's Recombinant DNase I?

    Answer: Takara Bio's Recombinant DNase I is known for its high purity and activity while being RNase-free. It delivers reliable and reproducible results, making it an excellent choice for genomic and cDNA library preparation, RNA analysis, and more. Its formulation helps maintain enzymatic activity across various buffers and conditions. This makes it highly suitable for laboratories aiming for accuracy in nucleic acid studies. Researchers appreciate its consistency, which often translates into valuable time savings during experimental procedures.
  • Question: Does Recombinant DNase I RNase-Free require special handling?

    Answer: Recombinant DNase I RNase-Free does not require extensive special handling beyond standard laboratory practices. However, to prevent contamination, it's crucial to use sterile techniques and gloves when working with the enzyme. Avoid common contaminants that could degrade your samples, such as RNase contamination from non-sterile surfaces. Ensure that you re-cap tubes promptly after use and use dedicated pipette tips. Following these practices maximizes the reliability of your results and the quality of your RNA preparations.
  • Question: Where can I buy Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A in Rwanda?

    Answer: You can purchase Recombinant DNase I RNase-Free, Takara Bio Cat. No. 2270A from Ubuy in Rwanda. Ubuy offers a broad selection of laboratory reagents and instruments, including high-quality enzyme products from reputable brands like Takara Bio. Simply visit the Ubuy website, search for the product by its catalog number, and place your order conveniently.
  • Question: What makes Takara Bio's Recombinant DNase I unique compared to competitors?

    Answer: Takara Bio's Recombinant DNase I stands out due to its exceptional purity and targeted RNase-free formulation, which minimizes the risk of RNA degradation during use. This feature enables researchers to perform nucleic acid experiments with confidence, reducing the potential of contamination that could lead to compromised results. Moreover, Takara Bio is known for maintaining stringent quality control processes, ensuring that their products consistently meet high standards in the laboratory. Thus, choosing their product often yields more reliable and reproducible experimental outcomes.

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Pros

  • Highly effective at DNA digestion
  • RNase-free for reliable performance
  • Purity ensures optimal results
  • Easy to use and handle
  • consistent performance across batches

Cons

  • Requires careful storage conditions

Product Price History

Important information

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Features & Benefits

  • Endonuclease for effective DNA degradation.
  • Works equally on single- and double-stranded DNA.
  • Produces 5'-P terminal oligonucleotides.
  • RNase-free for contamination-free results.
  • Stable at optimum neutral pH for optimal performance.
  • Ideal for RNA preparation purposes.