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Item #:
15629307
HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic Acid)], 100 Grams
85% of respondents would recommend this to a friend
Item #:
15629307
RWF 195155
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Chemical compound with a pKa range of 7.45-7.65 at 20°C and a pH range of 6.8-8.2.
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What Stands Out
High Purity
Our HEPES sodium salt offers exceptional purity, ensuring reliable performance in biological and biochemical applications without unwanted contaminants interfering with results.
Versatile Buffering
Designed for versatile buffering capacities, HEPES is ideal for maintaining stable pH in various lab settings, enhancing protocol accuracy, and improving overall experimental outcomes.
Convenient Packaging
Packaged in a 100-gram quantity, this product provides ample supply for extensive use in research, facilitating easy integration into your laboratory workflow.
Product Details
- Sold for research or further manufacturing use only, not for food or drug use
- Chemical Formula: C8H17N2O4SNa
- CAS Number: 75277-39-3
- Molecular Weight: 260.3
- Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
- pKa (20°C): 7.45-7.65, pH Range: 6.8-8.2
| Container Type | Bottle |
| Item Weight | 2.24 ounces (63.5 grams) |
| Brand Name | RPI |
| Manufacturer | RPI, Corp. |
| Part Number | H75050-100.0 |
| Brand | RPI |
| Package Information | Bottle |
Dangerous Goods
To avoid the delay in customs clearance process for Dangerous Goods, the shipment shall be opened at our warehouse facility to check the severity of hazardous components. Please see the list of Dangerous goods here.
Who Should Buy?
-
Laboratory Researchers
Ideal for researchers needing a buffering agent in biochemical and molecular biology experiments involving various pH levels.
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Cell Culture Practitioners
Highly suitable for cell culture applications where stable pH is essential for optimal cell growth and maintenance.
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Biotechnology Students
Valuable resource for students learning about pH buffers and their applications in experimental biology and biotechnology labs.
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Non-Scientific Use
Not suitable for casual users or those without proper scientific training or understanding of biochemicals.
Product Description
HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic Acid)], 100 Grams
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Customer Questions & Answers
-
Question:
What is HEPES, Sodium Salt used for?
Answer: HEPES, Sodium Salt, is primarily utilized as a buffering agent in biological and biochemical applications. It maintains the pH levels in cell culture and other laboratory experiments where stable pH is crucial for reactions, especially in the presence of carbon dioxide. Additionally, due to its effective buffering capacity around physiological pH, it's commonly employed to prepare media for cell growth, ensuring optimal conditions for cellular processes. -
Question:
How does HEPES compare to other buffers?
Answer: HEPES offers several advantages over traditional buffering agents like phosphate buffers. It is less toxic to cells, making it preferable for cell culture work. Additionally, HEPES remains stable across a wide pH range and is not significantly affected by the presence of proteins, unlike many other common buffers. This unique stability ensures reliable experimental outcomes in various biochemical analyses, from enzyme assays to protein studies. -
Question:
Is HEPES pH stable at room temperature?
Answer: Yes, HEPES is well-known for its stability in maintaining pH across a range of temperatures, including room temperature. This characteristic makes it ideal for experiments conducted outside refrigeration, providing consistent results without the risk of pH drift. Researchers often utilize HEPES solutions for experiments that may take several hours or days, ensuring that the formulated conditions remain constant throughout. -
Question:
What precautions should be taken when handling HEPES?
Answer: When handling HEPES, it’s important to follow standard laboratory safety protocols such as wearing gloves and goggles. HEPES is generally safe, but exposure to large amounts can cause irritation. Always work in a well-ventilated space, and avoid ingestion or inhalation. Proper storage in a cool, dry place is vital to prevent degradation, ensuring that your reagent remains effective for various applications, from research to product formulation. -
Question:
Can HEPES be used in mammalian cell culture?
Answer: Absolutely, HEPES is widely used in mammalian cell culture due to its excellent buffering capacity near physiological pH. It helps stabilize pH levels, which is critical because many cellular functions are pH-dependent. Researchers often add HEPES to their culture media, particularly for experiments that require CO2-independent conditions, allowing for more accurate and reliable cell assessments. -
Question:
What is the solubility of HEPES in water?
Answer: HEPES is highly soluble in water, with a solubility of approximately 1 gram per milliliter at room temperature. This solubility ensures that it can easily be incorporated into various aqueous solutions for use in diverse scientific applications. When preparing solutions, it’s recommended to add HEPES gradually while stirring to achieve complete dissolution, which contributes to a homogenous and effective buffering solution. -
Question:
Is HEPES suitable for protein studies?
Answer: Yes, HEPES is an excellent choice for protein studies as it provides a stable pH environment crucial for maintaining protein structure and function during experiments. Its inertness means it does not interfere with most biochemical assays, making it an ideal buffering agent for enzyme kinetics, protein purification, and stability assays. Researchers favor HEPES for applications that require rigorous conditions, ensuring experimental accuracy and reproducibility. -
Question:
Can HEPES be used in diagnostic tests?
Answer: HEPES is increasingly utilized in diagnostic tests, particularly in assays that require strict pH control for optimal performance. Its application in enzyme-linked immunosorbent assays (ELISAs) and other biochemical tests showcases its versatility. By maintaining consistent pH levels, HEPES ensures reliable enzyme activity during testing, improving the accuracy of results in clinical laboratories. -
Question:
What are some common formulations that include HEPES?
Answer: Common formulations containing HEPES include culture media for mammalian cells, buffers for protein assays, and solutions used in electrophoresis. HEPES is often included in F-10 and DMEM media for cell growth, and in various buffers designed for biochemical assays. Researchers utilize HEPES not only for its buffering capacity but also for its compatibility with numerous biological components, helping in the preparation of effective experimental assays. -
Question:
Where can I buy HEPES, Sodium Salt [N-2-Hydroxyethyl piperazine-N'-2-ethanesulfonic Acid], 100 Grams?
Answer: You can conveniently buy HEPES, Sodium Salt from Ubuy in Rwanda. Ubuy is a trusted e-commerce platform that offers a wide range of chemical products, making it a reliable source for researchers and labs seeking high-quality reagents. By choosing Ubuy, you can ensure a seamless shopping experience and access to product specifications necessary for your scientific applications.
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Pros
- High purity for reliable results
- Excellent buffering capacity
- Stable pH in biological systems
- Versatile application in research
- Convenient 100 grams packaging
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